Analysis Note

ControlCONTROL ANTIGEN: Included free of charge with the antibody is 120 µg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For positive control, in Western blot using 20 ng of protein per lane (minigel). For negative control, preincubate 3-5 µg of fusion protein with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Detect Potassium Channel Kv1.3 using this Anti-Potassium Channel Kv1.3 Antibody validated for use in IH & WB.

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-Potassium Channel Kv1.3 Antibody detects level of Potassium Channel Kv1.3 & has been published & validated for use in IH & WB.

Immunogen

GST fusion protein and a C-terminal portion of human Kv1.3 protein (amino acids 471-523) (Accession P22001).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB9784

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 mL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Format: Purified

Specificity

Recognizes a full length Kv1.3 protein. Possibly cross reacts with Kv1.1.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.